IKAN TRANSGENIK PDF

IDENTIFIKASI IKAN CUPANG (Betta imbelis) TRANSGENIK FOUNDER MEMBAWA GEN PENYANDI HORMON PERTUMBUHAN; Identification of Transgenic. Ikan transgenik F-0 dibuat dengan menggunakan metode transfeksi. Identifikasi dilakukan menggunakan metode RT-PCR. RNA total. Identification of zygosity in transgenik fish is normally achieved by PCR analysis with genomic DNA template extracted from the tissue of progenies which are.

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This technique could be useful in the establishment of breeding programs for mass transgenic fish production and in experiments in which zygosity effect could have a functional impact. In this experiment, we applied a quantitative real-time PCR qr-PCR method to analyze zygosity in a stable line of transgenic zebrafish Danio rerio carrying masu salmon, Oncorhynchus masou D6-desaturase-like gene. Tingkat ekspresi transgen yang tinggi pada jaringan ikan F2 yang diperiksa telah diperoleh.

The transmission rate of transgene into F1 generation was 4.

Poster Ikan Lele Transgenik by yadi apriadi on Prezi

Expression of transgene in F2 generation was varied between strains regarding as the mosaics of F0 fish. Jurnal Akuakultur Indonesia Vol 2, No 1 Metode ini memerlukan ikan dalam jumlah yang banyak, dan juga waktu serta tenaga. Rapid method for identification of transgenic fish zygosity Jurnal Akuakultur Indonesia Vol 6, No 2 Jurnal Akuakultur Indonesia Publisher: Transgen telah ditransmisikan ke ikan generasi F2 dengan mengikuti ikxn segregasi Mendel. Pendekatan baru untuk mengatasi masalah tersebut akan memberikan manfaat besar kepada peneliti bioteknologi perikanan.

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Teknik ini telah diaplikasikan pada transgenki yang memiliki nilai ekonomis. This method needs relatively large amounts of fish material and is time- and labor-intensive.

New approaches addressing this problem could be of great help for fish biotechnologists. Jurnal Akuakultur Indonesia Vol 6, No 2 Aquacultural output will need to be increased several fold in order to meet the rising demands for fish in coming years as the increasing of mankind population.

Identification of zygosity in transgenik fish is normally achieved by PCR analysis with genomic DNA template extracted from the tissue of progenies which are derived by mating the transgenic fish and wild-type counterpart. Mating of those transgenlk with wild-type had revealed transgene transmission to the offspring following expected Mendelian laws.

JURNAl AQUACOASTMARINE

Salah satu teknik modern yang diduga akan menjadi sarana yang berguna dalam pengembangan akuakultur adalah teknologi transfer gen. Recently, global food security has become a hot issue by the public in national as well as out of the country. The first F1 and second F2 generation was produced by crossing the transgenic founder F0 and F1, respectively, with wild-type fish. Now, a transgenic system to study the modification of fatty acid biosynthesis pathways in fish was established.

Ulasan ini menggambarkan variasi metode transfer gen, persistensi dan ekspressi dari gen yang ditransfer, aplikasi dan prospeknya ke depan dari penelitian transfer gen dalam akuakultur. Jurnal Akuakultur Indonesia Vol 6, No 1 Persilangan antara ikan transgenik tersebut dengan ikan normal menunjukkan transmisi transgen ke keturunannya mengikuti hukum segregasi Mendel.

Data dianalisis menggunakan metode pembandingan nilai cycle threshold. Thus, we found that the qTR-PCR to be effective for trnsgenik rapid and precise determination of zygosity in transgenic fish. The intensity and capacity of production is expected to increase using trandgenik approach. Data were analyzed using the comparative cycle threshold method. Highly unsaturated fatty acids HUFAespecially eicosapentaenoic acid EPA and docosahexaenoic acid DHA have long been recognized for its beneficial effect for human health and development.

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Teknik ini dapat berguna dalam program produksi ikan transgenik secara massal dan dalam percobaan dimana faktor sigositas memberikan pengaruh nyata. Produksi akuakultur diharapkan dapat ditingkatkan beberapa kali lipat untuk memenuhi kebutuhan pangan berupa ikan dimasa-masa mendatang akibat peningkatan populasi manusia.

Dengan demikian, metode krt-PCR adalah efektif untuk penentuan sigositas secara cepat dan tepat pada ikan transgenik. Intensitas dan kapasitas produksi diharapkan meningkat dengan menggunakan pendekatan bioteknologi.

The D6 fatty acid desaturase is generally considered to be the rate-limiting factor in HUFA biosynthesis. This Author published in this journals. This review describes various techniques of gene transfer, persistence and expression of transferred gene, application and future aspect of gene transfer research in aquaculture.

One of the advances biotechnologies that expected to be a powerful approach for aquaculture development is transgenic technique. Dengan demikian, sebuah sistem transgenik untuk memodifikasi biosistesa asam lemak pada ikan telah dikembangkan. This technique has been applied to several commercially valuable species.